Monitoring generic E. Coli based on lateral flow biosensor for water fecal pollution assessment скачать в хорошем качестве

Monitoring generic E. Coli based on lateral flow biosensor for water fecal pollution assessment

ICN2 Severo Ochoa Workshop on Environmental Monitoring and Remediation Title: Monitoring generic Escherichia coli based on lateral flow biosensor for water fecal pollution assessment Speaker: Liming Hu (ICN2) Abstract: Microbiological pollution is one of the important threats for good water quality worldwide. Escherichia coli (E. coli) is a good indicator for monitoring water microbiological pollution due to two main reasons: first, E. coli is the most abundant bacteria in mammal digestive systems; and second, E. coli can be also more easily grown and detected than other water-borne pathogens (i.e. other bacteria and viruses)1 . The traditional gold standard methods to detect E. coli (filtration membrane and direct bacteria cultivation) are time-consuming (over 24 h), expensive, well-equipped and complicated, which hinder in-situ detection and in-the-field analysis2 . Lateral flow assay offers several advantages over the aforementioned technologies, such as rapidity, simplicity, low-cost, easy interpretation, easy usage and mass screening on site3 . In this work, we firstly utilized the bioluminescent bacteria (Allivibrio fischeri) to analogously characterize the fluidity of E.coli on different size of nitrocellulose membrane and rapidly choose the optimal membrane, and optimized the size of gold nanoparticles, the concentration of antibody conjugated on nanoparticles and concentration of capture antibody sprayed on membrane and then developed a polyclonal antibody-based lateral flow biosensor (LFB) with an easily-interpretable colorimetric output to detect “general” E. coli. Finally, under the optimal conditions, a sensitivity of 104 CFU/mL in river water samples was achieved by combining the LFB with a filtration system within a total time of 30 minutes with a good reproducibility (RSD: intra-assay under 10%, inter-assay under 15%)4 . ----------------------------------------------------------------------------------------- The workshop on Environmental Monitoring and Remediation brought together leading academic scientists, researchers, scholars and industry representatives to exchange and share their experiences and results on all the aspects involved in pollutant detection, monitoring, scavenging and degradation. The workshop topics also covered the life-cycle of nanomaterials as potential pollutants and their impact on the ecosystem. This Action is held within the framework of the 2018-2021 Excelencia Severo Ochoa Programme. The event, which took place at the Casa de Convalescencia in Barcelona on December 16-17 2021, was organised by Dr Neus Bastús, CSIC Tenured Scientist in the Inorganic Nanoparticles Group, Dr Maria José Esplandiu, CSIC Scientific Researcher in the Magnetic Nanostructures Group, and Jessica Casandra Ramírez, Doctoral Student in the Magnetic Nanostructures Group. Event website: https://icn2.cat/en/severo-ochoa-work... News item about the event: icn2.cat/en/news/4799-icn2-severo-ochoa-workshop-on-environmental-monitoring-and-remediation