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Watch the Full Video at https://www.jove.com/v/57131/a-burrow.... A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae - a 2 minute Preview of the Experimental Protocol Karen M. Qiang, Fanli Zhou, Kathleen M. Beckingham Rice University, Department of Biosciences; Yale Medical School,; The protocol describes a simple assay to identify Drosophila melanogaster larvae that are experiencing hypoxia under normal atmospheric oxygen levels. This protocol allows hypoxic larvae to be distinguished from other mutants that show overlapping phenotypes such as sluggishness or slow growth. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments