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Watch the Full Video at https://www.jove.com/v/1267/generatio.... Generation of Single-Cell Suspensions from Mouse Neural Tissue - a 2 minute Preview of the Experimental Protocol Sandra Pennartz, Sandy Reiss, Rebecca Biloune, Doris Hasselmann, Andreas Bosio Miltenyi Biotec,GmbH,; Dissociating cells from specific tissue types requires specific parameters for tissue aggitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS Dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on nerual tissue is explained. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments