GN 311 Mini Video 4-1B скачать в хорошем качестве

GN 311 Mini Video 4-1B

DNA Coding Strand 5' - ATG TGG AAC GCT TGC TGA CCC GGC - 3' mRNA Sequence 5 - AUG UGG AAC GCU UGC UGA Met Trp Asn Ala Cys STOP the UGA codon codes for the stop codon and translation stops Missense: change in amino acid This example: (AAC(from original at top) to AGC(mutant))(Asn to Ser) *Adenine(purine) to Guanine(purine) -- Transition DNA Coding Strand 5' - ATG TGG AGC GCT TGC TGA CCC GGC - 3' mRNA mutant 5' - UTG UGG AGC GCU UGC UGA Met Trp Ser Ala Cys STOP the UGA codon codes for the stop codon and translation stops Nonsense: change in base that leads to codon coding for stop codon This example:(TGC(from original at top)to TGA(mutant))(Cys toSTOP) *Cytosine(pyrimidine) to Adenine(purine) -- Transversion DNA Coding Strand 5' - ATG TGG AAC GCT TGA TGC CCC GGC - 3' mRNA mutant 5' - UTG UGG AAC GCU UGA Met Trp Asn Ala STOP the UGA codon codes for the stop codon and translation stops Silent: change in base where the new codon codes for the same amino acid This example: (TGC (from original at top)to TGT(mutant)) (Cys to Cys) *Cytosine(pyrimidine) to Thymine(pyrimidine) -- Transition DNA Coding Strand 5' - ATG TGG AAC GCT TGT TGA CCC GGC - 3' mRNA mutant 5' - UTG UGG AAC GCU UGU UGA Met Trp Asn Ala Cys STOP the UGA codon codes for the stop codon and translation stops Readthrough: the base change occurs within the stop codon and the new codon codes for an amino acid. Translation continues. This example:(TGA(from original at top)to TGG(mutant))(STOP to Trp) *Adenine(purine) to Guanine(purine) -- Transition DNA Coding Strand 5' - ATG TGG AAC GCT TGC TGA CCC GGC - 3' mRNA mutant 5' - UTG UGG AAC GCU UGC UGG CCC GGC -3' Met Trp Asn Ala Ala Trp Pro Gly the UGA stop codon becomes an amino acid (Trp) and translation proceeds