Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes l Protocol Preview скачать в хорошем качестве

Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes l Protocol Preview

Watch the Full Video at https://www.jove.com/v/4054/a-parasit.... A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line - a 2 minute Preview of the Experimental Protocol Surendra K. Jain, Rajnish Sahu, Larry A. Walker, Babu L. Tekwani University of Mississippi, National Center for Natural Products Research, School of Pharmacy; University of Mississippi, Department of Pharmacology; A parasite-rescue and transformation assay with THP1 cells infected in vitro with Leishmania donovani has been optimized for anti-leishmanial drug screening. The assay involves differentiation of THP1 cells, infection with promastigotes, treatment with test drugs, controlled lysis of the infected macrophages, rescue of amastigotes, transformation to promastigotes and monitoring promastigote growth and proliferation with a fluorometric assay. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments