The Cell Biology of Extracellular Vesicles: From Immunity  to Pigmentation by Graça Raposo скачать в хорошем качестве

The Cell Biology of Extracellular Vesicles: From Immunity  to Pigmentation by Graça Raposo

WebEVTalk 011 Graça Raposo (Research Director in Institut Curie, CNRS, France) gives a lecture on the cell biology of extracellular vesicles, how they are involved in immunity and pigmentation. The lecture covers the history of the field of extracellular vesicles (EVs), the discovery of melanosomes and multivesicular bodies, and the characterization of the cellular machinery involved in these processes. #WebEVTalk #ExtracellularVesicles #CellBiology #Immunity #Pigmentation #EVs Hosts: Dr Carolina Soekmadji (Australia) Prof Jan Lotvall (Sweden) Early evidence of EVs from Phil Stahl’s lab (Harding et al., 1984). https://europepmc.org/article/med/615... Early evidence of EVs from Rose Johnston’s lab (Pan et al., 1985). https://rupress.org/jcb/article/101/3... The first report of exosomes as intraluminal vesicles of multivesicular endosomes that are secreted upon fusion of these endosomes with the plasma membrane, coining the term ‘exosomes’ (Johnstone et al., 1987). https://www.jbc.org/content/262/19/94... Exploiting electron microscopy, biochemistry and functional assays, this manuscript shows for the first time that antigen-presenting cells secrete exosomes able to stimulate T cell proliferation (Raposo et al., 1996). https://rupress.org/jem/article/183/3... (Zitvogel et al., 1998). https://www.nature.com/articles/nm059... (Fevrier et al., 2004). https://www.pnas.org/content/101/26/9... The first exhaustive and detailed protocol providing isolation and characterization procedures of exosomes present in cell culture supernatants and biological fluids (Théry et al., 2006). https://currentprotocols.onlinelibrar... Demonstration that exosomes contain mRNAs and miRNAs and mediate transfer between cells (Valadi et al., 2007). https://www.nature.com/articles/ncb1596 (Gould and Raposo, 2013). https://www.tandfonline.com/doi/full/... The first report showing a mechanism for microvesicle release through the involvement of ARF6 and cytoskeletal rearrangements. It also shows that microvesicles but not exosomes carry metalloproteases able to digest the extracellular matrix (Muralidharan-Chari et al., 2009). https://www.sciencedirect.com/science... This study reports that upon interaction with T cells, dendritic cells target MHC II molecules towards exosome secretion by using a multivesicular body pathway distinct from those employed by dendritic cells to target MHC II molecules towards lysosomal degradation (Buschow et al., 2009). https://www.ncbi.nlm.nih.gov/pubmed/1... (Van Niel et al., 2011). https://www.sciencedirect.com/science... (Harding et al., 1983). https://rupress.org/jcb/article/97/2/... (Raposo and Stoorvogel, 2013). https://rupress.org/jcb/article/200/4... (Cicero et al., 2015b). https://www.sciencedirect.com/science... (Van Niel et al., 2015). https://www.sciencedirect.com/science... (Cicero et al., 2015a) https://ui.adsabs.harvard.edu/abs/201... Study showing that exosomes can be released as clusters that remain attached to each other and anchored to the cell surface by tetherin. It suggests that the tetherin-mediated attachment may have a key role in exosome fate (Edgar et al., 2016). https://elifesciences.org/articles/17180 (Verweij et al., 2018). https://rupress.org/jcb/article/217/3... (Verweij et al., 2019). https://www.sciencedirect.com/science... (Stahl and Raposo, 2019). https://journals.physiology.org/doi/f... (Raposo and Stahl, 2019). https://journals.physiology.org/doi/f... (van Niel et al., 2018). https://www.nature.com/articles/nrm.2... (Théry et al., 2018). https://www.tandfonline.com/doi/full/...