mRNA manufacturing platform using CIMmultus® and CIMac® technology with rapid HPLC analytics скачать в хорошем качестве

mRNA manufacturing platform using CIMmultus® and CIMac® technology with rapid HPLC analytics

Cell & Gene Therapy Insights' FastFacts video Published: December 2, 2021 Watch the video to learn about: Non-affinity chromatography with PrimaS provides purification for IVT processes with co-transcriptional polyadenylation The Sartorius mRNA purification toolbox provides a scalable chromatographic solution for commercial manufacturing of mRNA therapeutics CIMac analytical HPLC columns can resolve individual IVT reaction components in minutes, resolving the analytical bottleneck in optimization of IVT reaction conditions Rapid HPLC analytics can improve in-process and quality control of the mRNA production process GET in TOUCH: Webpage: https://www.biaseparations.com/ E-mail: sales@biaseparations.com Tech support: help@biaseparations.com CHAPTERS: 0:00 Introduction 0:11 Mature mRNA structure 4:24 Chromatography media selection for mRNA (and pDNA) 7:36 Different purification and analytical approaches, different ligands 09:20 Affinity Capture with CIMmultus oligo dT18 11:03 Multimodal capture of mRNA without Poly-A tail with CIMmultus PrimaS 12:29 mRNA polishing - C4 HLD 14:15 mRNA polishing - SDVB 15:05 Different purification and analytical approaches, different ligands 15:35 CIMac PrimaS: Quantification of mRNA, NTPs, pDNA and Capping Reagent in IVT 17:15 CIMac PrimaS: Capping efficiency (with enzymatic digestion) 18:12 IPC analytics: Detection of "non-ssRNA" 19:09 Case study: mRNA stability