Importance of Gel Equilibration for Western Blotting | Equilibrating the Gel/Preparing for Transfer скачать в хорошем качестве

Importance of Gel Equilibration for Western Blotting | Equilibrating the Gel/Preparing for Transfer

Following the gel run, we can prepare for the transfer step. A successful Western blot depends on efficient transfer of your protein from gel to membrane while maintaining resolution and retention of your protein. After running the gel, the first step is to equilibrate the gel in transfer buffer. Gel equilibration is a commonly overlooked step. Failing to equilibrate your gel in transfer buffer can negatively impact the transfer of your proteins from gel to membrane. About LICORbio LICORbio offers imaging systems, analysis software, and IRDye® infrared dye reagents for quantitative protein and small animal imaging. LICORbio provides an integrated solution for quantitative Western blots with its Odyssey® Infrared Imaging Systems, Image Studio™ quantification software, Empiria Studio® analysis software for publication-ready data, reagents, unique IRDye® secondary antibodies, and online training courses. For chemiluminescent Western blots, the C-DiGit® Blot Scanner is a personal, portable imager. Use the Odyssey® XF or Odyssey M Imaging Systems for both chemiluminescence and near-infrared fluorescence Western blots. You can achieve superior small animal imaging results using BrightSite™ near-infrared fluorescent probes or bioluminescence on the Pearl® Trilogy imaging system. LICORbio Website https://www.licorbio.com/ Reagent Store https://www.licor.com/bio/reagents Image Studio™ Software https://www.licor.com/image-studio LinkedIn   / licorbio   Twitter   / licorbio   Facebook   / licorbio