Understand Electroporation In Under 3 Minutes скачать в хорошем качестве

Understand Electroporation In Under 3 Minutes

Electroporation Simply Explained Electroporation is a physical transfection method, in other words it is a method to artificially introduce nucleic acids (DNA or RNA) into cells. In the case of electroporation this is done with the help of an electrical pulse which creates small temporary holes or pores in the phospholipid bilayer, or THIS part i.e. the cell wall. This is done in 4 steps. First the host cells plus the nucleic acids we wish to insert are placed in a conductive solution. An electrical circle is enclosed around the mixture. Second, an electrical pulse of optimized timing and voltage is applied across the solution. Third, this disturbs the phospholipid bilayer, creating small holes or pores in it. Simultaneously this charge creates an electric potential between the outside and inside of the cell forcing the nucleic acids through the pores and inside since these are negatively charged. Fourth and finally, the cell is allowed to recover and hopefully regenerate its phospholipid bilayer. Electroporation is advantageous since it is easy, rapid and stable. It can transfect a large number of cells in a short time and is applicable for all cell types. However, its major disadvantage is the substantial cell death it incurs due to the high voltage pulse which often leaves the cells unable to fully recover.