Webinar: Make the Biggest Impact - Optimise Your High-Throughput Screening with the CERTUS FLEX скачать в хорошем качестве

Webinar: Make the Biggest Impact - Optimise Your High-Throughput Screening with the CERTUS FLEX

As part of AstraZeneca’s new global HQ and R&D centre at the Cambridge Biomedical Campus, AstraZeneca’s Centre for Lead Discovery (UKCLD) contains world leading infrastructure for the storage and testing of our collection of over 2 million compounds. Currently AstraZeneca have twelve automated CERTUS FLEX Liquid Dispensers on CoLAB Flex carts, and four dispensers for use offline. We chose the CERTUS FLEX for dispense speed, accuracy and lower dead volumes (the use of syringe-based dispensing reduces dead volumes to 120 µl), although our initial evaluation also highlighted enhancements to make it suitable for uHTS screening. In collaboration with the Fritz Gyger AG, we developed a more flexible dispenser to meet our uHTS needs, for example, a software to allow simultaneous dual addition of reagents, using up to eight valves in parallel per reagent. We also developed an angled dispense head, of benefit for both cell-based assays (reducing the risk of disrupting cell monolayers after washing steps) and biochemical assays (reducing bubbles/frothing and eliminating the need for centrifugation post-addition). Together we have also developed peripherals for the CERTUS FLEX, such as a universal bottle holder and increased volume reagent bottles for HTS volumes. We continue to work with Fritz Gyger AG on CERTUS FLEX product developments, including an offline valve cleaning station, which will be launched this month and an automated valve auto calibration/verification procedure utilising HighRes Cellario software.