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Watch the Full Video at https://www.jove.com/v/56086/pulling-.... Pulling Membrane Nanotubes from Giant Unilamellar Vesicles - a 2 minute Preview of the Experimental Protocol Coline Prévost, Feng-Ching Tsai, Patricia Bassereau, Mijo Simunovic PSL Research University, CNRS UMR168, Laboratoire Physico Chimie Curie, Institut Curie; Harvard Medical School, Department of Genetics and Complex Diseases, T. H. Chan School of Public Health; Harvard Medical School, Department of Cell Biology; UPMC University Paris 06, Sorbonne Universités; The Rockefeller University, Center for Studies in Physics and Biology; Many proteins in the cell sense and induce membrane curvature. We describe a method to pull membrane nanotubes from lipid vesicles to study the interaction of proteins or any curvature-active molecule with curved membranes in vitro. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments