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Watch the Full Video at https://www.jove.com/v/57241/high-thr.... High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method - a 2 minute Preview of the Experimental Protocol Morgan A. Wambaugh, Jessica C. S. Brown University of Utah, Microbiology and Immunology Division, Department of Pathology; Synergistic drug combinations are difficult and time-consuming to identify empirically. Here, we describe a method for identifying and validating synergistic small molecules. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments