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When viewing multiplex or highplex Immunofluorescence scans, the coexpression of markers yields insights on present immune cell subpopulations. While the Cell based Colocalization App can be used to analyze the coexpression on a cell-by-cell level, the coexpression scatter plots presented in this video provide an instantaneous feedback on colocalizing markers. The interactive scatter plots show a 2D histogram where two channels’ intensities are plotted on the x- and y-axis. In the top left corner, the overall Pearson’s Correlation Coefficient (PCC) is displayed. The PCC ranges from -1.0 to 1.0. The higher absolute magnitude, the higher the correlation. Plots are automatically sorted by descending PCC. By clicking into the canvas, the canvas is subdivided into four quadrants. For each of the quadrants the relative amount of pixels that fall into that quadrant is computed as well as the PCC. By clicking and dragging, an additional arbitrarily placed rectangular ROI can be drawn. This App is only available in MIKAIA studio, not in MIKAIA lite. Download: https://www.micaia.ai Bleib mit uns in Kontakt: Website: https://www.iis.fraunhofer.de Facebook: / fraunhoferiis Twitter: / fraunhoferiis LinkedIn: / fraunhofer-iis XING: https://www.xing.com/companies/fraunh... #FraunhoferIIS MICAIA was recently renamed MIKAIA.