Nucleofection for Efficient Transfection of Human THP-1 Macrophages | Protocol Preview скачать в хорошем качестве

Nucleofection for Efficient Transfection of Human THP-1 Macrophages | Protocol Preview

Watch the Full Video at https://www.jove.com/v/51960/highly-e.... Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection - a 2 minute Preview of the Experimental Protocol Marten B. Maeß, Berith Wittig, Stefan Lorkowski Friedrich Schiller University Jena, Institute of Nutrition; This protocol presents an efficient and reliable method to transfect human THP-1 macrophages with siRNA or plasmid DNA by electroporation with high transfection efficiency while maintaining high cell vitality and full macrophage capacity for differentiation and polarization. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments