How to Find the Perfect Sonicator for Cell Disruption and Protein Extraction in Science and Analysis скачать в хорошем качестве

How to Find the Perfect Sonicator for Cell Disruption and Protein Extraction in Science and Analysis

For more information, please contact us or visit https://www.hielscher.com/lab ! Welcome to this presentation, where we'll uncover the fundamentals behind finding the ideal sonicator for cell disruption and protein extraction, along with its various applications in science and analysis laboratories. Why are sonicators the preferred choice for cell lysis and protein extraction? Their effectiveness, versatility, and gentle approach set them apart from other methods. Think of sonication as a method that gently yet effectively breaks open cells. It employs intense ultrasound waves to create bubbles, which lead to mechanical stress. This stress breaks cell walls, letting their contents out. This is especially helpful when dealing with sensitive cells or proteins that can be harmed by harsh methods. With Hielscher sonicators, you can control the process, making it fit each sample's characteristics. Sonicators, also known as ultrasonic tissue homogenizers, have multiple roles. They use ultrasound waves to gently open cells, isolate molecules inside them, and even break DNA. Researchers and lab technicians use them for tissue homogenization, cell lysis, protein extraction, fractionation, and DNA fragmentation. In research and analysis labs, a variety of containers are used to hold samples. The choice of container depends on ensuring proper handling, analysis, and storage of the cellular material. Each container has specific properties like material, size, shape, and design. These properties guide the selection of the best container for each unique sample process. With countless types of containers used daily in labs, there are tailored procedures that match the container type. Moreover, the characteristics of the sample material, such as cells and tissues, must also be considered when choosing the best preparation method. If your work often involves closed vials like Eppendorf tubes, cryo-vials, autosampler vials, or similar test tubes, the VialTweeter sonicator could be your perfect solution (see https://www.hielscher.com/vialtweeter). Designed for indirect sonication of up to 10 sample tubes simultaneously, the VialTweeter simplifies sample preparation and reduces repetitive tasks. During the entire sonication process, the vials and test tubes can remain sealed. As no ultrasonic probe is inserted into the liquid, the risk of cross-contamination and sample loss is eliminated. Simply place up to 10 test tubes into the openings of the VialTweeter block and select your desired process settings on the ultrasonic generator's digital touch screen. Lab technicians also appreciate the sonicator’s versatility in handling diverse sample types. They accommodate everything from soft tissues and gram-negative bacteria to tougher materials like solid tissue samples from heart, lung, liver, muscle, and cartilage, or gram-positive bacteria. This adaptability makes sonicators valuable across various research areas. Furthermore, indirect sonication performed in sealed tubes or containers mitigates the risk of cross-contamination between samples. This feature proves particularly advantageous when working with pathogens or toxic materials. Whether you need to ultrasonically agitate a single vessel, a few test tubes, or multi-well plates for high-throughput lysis, Hielscher offers tailored solutions. Special sonicator models enable simultaneous processing of multiple samples, enhancing throughput and efficiency. As we conclude this presentation, it's evident that diverse sonicator types cater to efficient, reliable, and user-friendly sample preparation across different materials and quantities. Whether it's direct sonication of larger samples in open containers or indirect sonication of multiple samples using sealed tubes, Hielscher offers solutions that align with your needs. As you contemplate your next steps, remember that Hielscher experts are ready to guide you in selecting the ideal sonicator for your lab work. For any questions about specific sample types, applications, or protocols, our experienced team is here to provide comprehensive assistance. Don't hesitate to reach out. We eagerly await your message and look forward to partnering with you to achieve your goals. If you found this presentation informative, please consider liking this video. Subscribe to our channel to stay updated on our future videos! For more information, please contact us or visit https://www.hielscher.com/lab ! 0:00 Introduction 0:34 Sonication for Cell Disruption and Protein Extraction 1:23 Working Principle of Ultrasonic Lysis 2:11 Ultrasonic Cavitation 3:38 Ultrasonic Sample Preparation 6:53 Example: RNA Isolation 9:32 UIP400MTP 96-Well Plate Sonicator 12:29 Advantages of Ultrasonic Homogenizers 14:01 Temperature Control 15:33 Browser Remote Control