Using 3D Cell Models to Study Exercise and Breast Cancer | Dr Mhairi Morris, Loughborough University скачать в хорошем качестве

Using 3D Cell Models to Study Exercise and Breast Cancer | Dr Mhairi Morris, Loughborough University

Dr Mhairi Morris, Senior Lecturer at Loughborough University, explains how her research team uses 3D cell culture models to study the relationship between exercise, obesity, and breast cancer biology. Working within the School of Sport, Exercise and Health Sciences, Dr Morris is developing a 3D model of the obesogenic breast cancer microenvironment. The model incorporates fat cells from both visceral and subcutaneous sources to explore how exercise may differentially affect cancer cell viability in more physiologically relevant systems. To measure viability in these complex 3D cultures, the team uses CellTiter‑Glo® 3D, a luminescent assay that quantifies metabolically active cells through ATP detection. Dr Morris describes how the assay provides reproducible results when transitioning from traditional 2D cultures to 3D spheroids and organoids. The assay’s simplicity, short runtime, and ability to work directly in matrix‑embedded cultures have supported consistent measurement of viability across experiments, helping the team investigate how exercise may mitigate the effects of adipose cells within the tumour microenvironment. This video highlights the importance of accurate, reproducible viability measurements when studying cancer biology in advanced 3D models.