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https://www.thermofisher.com/us/en/ho... What is High-Content and is there a difference between the terms High-Content Screening and High-Content Analysis? * * What is high-content analysis and is there a difference between the terms high-content screening and high-content analysis? High-content analysis, or high-content screening, was a term created by Dr. Lans Taylor around 1996. High-content analysis refers to a methodology where individual cells are imaged and analyzed with an automated microscope system that produces a large number of individual cell measurements. This technique was possible prior to 1996, but it was in this year that Dr. Taylor formed Cellomics—now part of Thermo Fisher Scientific. Cellomics created the first platforms to fuse these technologies into a product that allowed scientists to scale high-content cell biology with higher resolution and greater assay sensitivity. The first applications of high-content screening were focused on drug discovery, but the technique later became known as high-content analysis in order to include scientists working across different areas. Terms like quantitative cell biology, quantitative imaging, and imaging cytometry are all terms for single cell analysis using images as a basis for detection. In parallel or as a separate step, image analysis software is used to examine and measure cellular changes. High-content screening or analysis is used interchangeably and continues to evolve as a technology that advances understanding of phenotypic change at the cellular level. Check out our other videos on frequently asked questions about high-content imaging.