NEW HUVEC Transfection Optimized with Cytofect™ Kit скачать в хорошем качестве

NEW HUVEC Transfection Optimized with Cytofect™ Kit

High definition live cell imaging (HD-LCI) to visualize real time expression of Green Fluorescent Protein (GFP) in human umbilical vein endothelial cells (HUVEC) after transient transfection using Cytofect™ HUVEC Transfection Kit (Cat. # TF200K, Cell Applications, Inc.). The HUVEC cells (Cat. # 200-05N, Cell Applications, Inc.) light up green due to the intracellular expression of GFP protein. Cytofect™ transfection reagent successfully delivered GFP-expressing vector into the cells, with protein detected as little as 3 hr post-transfection, compared to the 12+ hr often seen with traditional reagents. No significant cell death was observed post-transfection, a crucial indication of very low toxicity for potentially sensitive primary cells. The cells remained motile during the entire course of experiment, further indicating optimal cell health. Time-series imaging was performed in a humidified incubator set to 5% CO2 / 37°C using a Lumascope 620 microscope (Etaluma; www.etaluma.com). LED setup t = 1 sec, F2: lum=6%, g=112%, exposure = 869 units, 1 frame/2 mins. We found the Lumascope 620 offered high resolution and a small footprint that fit well in our standard-size incubator. The unit’s versatility included the ability to image through various cell culture vessels, and power supply via just its USB port.