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Cannabis is an extremely complex matrix that has chemical properties which can make the quantitation of various analytes within the product difficult. As such, laboratories must develop methods that will account for the matrix effect and interferences that naturally occur in everyday samples. While all analyses can be impacted by matrix effect, the analyses for pesticides and mycotoxins are impacted the most. This indicates that laboratories must take the matrix and resulting interferences into consideration when processing samples. If a laboratory wants to accurately identify and quantify compounds of interest, matrix calibrations, the use of internal standards, and robust method development are essential. This presentation will provide data and evidence regarding the impact that a matrix can play on pesticide and mycotoxin results and why the lack of matrix calibrations can be detrimental to the accurate quantitation of the analytes of interest. Additionally, tools for identifying matrix interferences and how to combat these issues will be discussed in further detail. Jini Curry is the Chief Scientific Officer at Modern Canna Labs in Lakeland, Florida. She received her bachelor’s degree with honors in Biochemistry from the University of West Florida and her master’s degree in Medical Cannabis Science and Therapeutics from the University of Maryland, Baltimore, becoming one of less than 350 people with this prestigious degree. Before entering the cannabis industry, Jini gained valuable research experience at several well-respected institutes, including Emory University, the National Institute of Aging, and the University of Florida. She began working with Modern Canna in 2016, moved into the laboratory director position in January 2018, and was recently promoted to chief scientific officer in August of 2022. Jini is extremely involved in the cannabis industry and currently serves on the Scientific Advisory Board for Analytical Cannabis, is a member of the CASP Proficiency Testing Advisory Task Force with AOAC International and is a member of the CannMed 2023 Advisory Board.