Analyzing full IgG antibody with paired VH/VL sequences скачать в хорошем качестве

Analyzing full IgG antibody with paired VH/VL sequences

Check out how to pair VH and VL chains during analysis to keep both chains together. Such input can be acquired from hybridoma sequencing of heavy and light chains sequenced separately in two different plates. After sequencing, the two chains can be paired together based on their identifier. Reference: Jain T, Sun T, Durand S, Hall A, Houston NR, Nett JH, Sharkey B, Bobrowicz B, Caffry I, Yu Y, Cao Y, Lynaugh H, Brown M, Baruah H, Gray LT, Krauland EM, Xu Y, Vásquez M, Wittrup KD. Biophysical properties of the clinical-stage antibody landscape. Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):944-949. doi: 10.1073/pnas.1616408114. Epub 2017 Jan 17. PMID: 28096333; PMCID: PMC5293111. Pipebio platform features configurable sequence & assay-data databases to structure data for machine learning and AI applications. Our strong analytical engine for large NGS datasets, REST API and intuitive tools for visualization are the foundations that form the integrated PipeBio bioinformatics platform. Interested in computational tools for antibody discovery? Get started for free - https://pipebio.com/sign-up Follow us to stay up to date:   / pipebio   Learn more about antibody research: https://pipebio.com/blog Chapters: 0:00 Introduction 0:11 Pipeline of analysis 0:36 Downloading data 1:32 Importing the data 2:12 Inspecting the sequences 2:27 Annotating sequences 3:07 Checking annotation results 3:46 Pairing annotated sequences