Developing glycoproteomics and glycoengineering approaches to investigate protein glycosylation скачать в хорошем качестве

Developing glycoproteomics and glycoengineering approaches to investigate protein glycosylation

Join us to discuss cutting-edge technologies and advances in the biomedical and life sciences! __ Protein glycosylation is one of the most abundant and the most complex post-translational modification (PTM) found in a cell. This complexity and diversity of glycan structures impose significant challenges to glycoproteome-wide analyses, that need to be approached with tailored methodologies. Analysis of PTMs at a proteome level is typically based on bottom-up mass spectrometry approaches, where proteins are subjected to protease digestion, separated by reverse-phase nano-liquid chromatography, subjected to ionization and tandem mass spectrometry analysis. In addition to these, glycan enrichment procedures are necessary for sensitive identification of glycopeptides among more abundant, non-glycosylated peptides. Additionally, the use of genome editing approaches allows simplification of complex and diverse glycan structures, allowing in this way their enrichment and mass spectrometric analysis. The rational inactivation and reintroduction of glyco-genes also enables dissection of (human) glycosylation pathways and their functions in cell biology. This Webinar introduces these and possible alternative strategies in the context of glycosylation analysis, as well as their applicability to specific research questions. The talk will refer to some of its applications primarily in the context of O-glycosylation, that enabled the characterization of - among others - O-mannosylation in human cells. Highlights 📌Overview of glycoproteomics strategies 📌Focus on genetic engineering, lectin chromatography and mass spectrometry 📌Suitable for different input samples-from purified proteins to cell/tissue extracts 📌Applications of this workflow to mammalian cells ❓ Share your question in the webinar Q&A ➡https://bio-protocol.org/webinar/deta... __ 🥼 About Bio-protocol Webinar Series The Bio-protocol webinar series highlights technical advances in biomedical and life science, focusing on a detailed presentation of cutting-edge technologies and techniques. We aim to make this program a go-to place for sharing, learning, discussing, and uncovering how to apply new technologies and tools in the life science community. 📩 To stay updated on our new webinars ➡ https://bio-protocol.org/webinar If this video was helpful, don't forget to like, subscribe, and share this video! For inquiries, contact us at: [email protected] Follow Bio-protocol! 🌿 Bluesky ➡https://bsky.app/profile/bio-protocol... 🔬 LinkedIn ➡ / bio-protocol