how to grow ergot скачать в хорошем качестве

how to grow ergot

this video is protected under fair use copyright law; for informational, educational and research purposes. ergot is isolated on a petri dish then transferred to liquid culture using malt extract agar for the petri dishes and light malt extract (or something similar) for the liquid cultures.. prepare a nutrient medium using malt extract agar.. "Dissolve in distilled water to make 1 liter. Adjust to Ph 4 with ammonium hydroxide solution to increase alkalinity or citric acid to increase acidity. Sterilize nutrient. This medium is placed in cotton-stoppered slants or in covered but not airtight petri dishes and inoculated with ergot. Temperature is maintained at 27°C; pH4 is maintained by periodic testing and adjustment. The culture is allowed to grow for 2 weeks under these conditions. After this time clusters of the fungus will be seen on the surface. 2) Large-Scale Production Construct aerated culture jugs as in the diagram below. http://postimg.org/image/f03fex3ip/ In a blender homogenize the activated ergot with its growing media. Fill all culture jugs 3/4 way with formula given above. Inoculate the jugs with portions of the homogenized active ergot culture. Keep jugs out of bright light at 25°C for 10 days with constant aeration. After 10 days adjust the culture to 1% ethanol by introducing 2 oz of 50/50 ethanol/water solution at injection point. Aeration must be momentarily stopped to do this. As soon as ethanol solution is added reconnect glass tubing to rubber tubing at injection point and resume aeration. Let growth continue for 14 more days. 3) Extraction After the total 24 days growth the culture is made acidic with tartaric acid solution and homogenized in a blender. After 1 hour adjust this to pH9 with ammonium hydroxide solution. Extract in a separation funnel with benzene or 50/50 chloroform/isobutanol. Extract again with alcoholic tartaric acid. Evaporate to dryness under reduced pressure. The dried product is the tartrate salt of the mixed ergot alkaloids. In this form it is fairly stable. It must be converted to the less stable free base before commencing synthesis of LSD-25. To do so adjust to pH9 with ammonium hydroxide, extract in chloroform and evaporate chloroform under reduced pressure." d-lysergic acid diethylamide LSD-25 rye ergot fungus sclerotium Ergine Hypocreaceae ergotamine ergopeptine Claviceps purpurea ; africana (sorghum); paspali ryegrass grass seed d-lysergic acid amide d-lysergamide alkaloid ergoline psychedelic seeds Turbina corymbosa ololiuhqui, Argyreia nervosa Hawaiian baby woodrose Ipomoea tricolor morning glories, tlitliltzin ergine isoergine synthetic synthesis LSA iso-LSA iso-LSD psychedelic Claviceps africana fusiformis paspali purpurea sorghi zizaniae lutea fungus