AAV Titration-Functional Assessment: Adeno- Virus Assembly-Activating Proteins l Protocol Preview скачать в хорошем качестве

AAV Titration-Functional Assessment: Adeno- Virus Assembly-Activating Proteins l Protocol Preview

Watch the Full Video at https://www.jove.com/v/56766/a-quanti.... A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins - a 2 minute Preview of the Experimental Protocol John M. Powers, Xiao Lan Chang, Zhen Song, Hiroyuki Nakai Oregon Health & Science University, Department of Molecular and Medical Genetics; Oregon Health & Science University, Department of Molecular Microbiology and Immunology; This manuscript details a straightforward dot blot assay for quantitation of adeno-associated virus (AAV) titers and its application to study the role of assembly-activating proteins (AAPs), a novel class of non-structural viral proteins found in all AAV serotypes, in promoting the assembly of capsids derived from cognate and heterologous AAV serotypes. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments