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Explore the science behind hit television show and video game The Last of Us in this AMA with mycologist and molecular biologist Jessie MacAlpine! (**Contains spoilers up to Episode 5 but the season one finale hadn't aired at the time of filming so this is a safe space 😉) Jessie is a PhD candidate in the Department of Molecular Genetics at the University of Toronto. Her research investigates the interactions between commensal bacteria and human fungal pathogens with the goal of discovering novel antifungal therapeutics. 0:00 - Introduction 1:22 - About The Last of Us 2:00 - What are cordyceps? 4:27 - Can fungal pathogens spread via physical contact? 6:07 - Why are mammals not infected? 8:18 - What's the temperature tolerance of fungi? 10:08 - How do cordyceps networks work in the real world? 12:20 - Slime mold vs. Cordyceps 14:05 - Fungi inspiration behind clickers 15:16 - Is food a likely vector for transmission? 17:30 - Why couldn't Ellie save Sam? 20:34 - Will it ever be possible for Cordyceps to infect people? 21:55 - What fungal pathogens should we be concerned about? 25:34 - What keeps you up at night? 27:26 - What’s your favourite thing about being a scientist? 28:23 - Conclusion If your questions weren't answered in the time we had, drop them in the comments below and Jessie will try to answer them over the next week or two!