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In a Richard Zsigmondy Lecture organised by the Austrian Academy of Sciences and Technical University Wien, Ondrej L. Krivanek gives an insight into the development of current electron microscopy, which makes it possible to analyse materials at the nano level. Ondrej L Krivanek is one of the major experts in electron microscopy and electron energy loss spectroscopy. He is co-founder an current President of Nion Co. and Affiliate Professor at Arizona State University. He has received numerous awards, including the Duddell Medal, the Prize of the British Institute of Physics, the Cosslett Medal from the International Federation of Microscopy Societies, and the Kavli Prize for Nanoscience. He is a fellow of the Royal Society, the Institute of Physics, the Microscopy Society of America, and of the American Physical Society, an honorary fellow of the Royal Microscopical Society, and holds an Honorary Doctorate of the Masaryk University, Brno in Czechia, and the University of Leeds in the UK. The Richard Zsigmondy Lecture series is organised by ÖAW and TU Wien and is dedicated to current issues at the interface of chemistry and microscopy. They commemorate the Austro-Hungarian chemist Richard Zsigmondy, who was a professor in Göttingen from 1908 and was awarded the Nobel Prize for Chemistry in 1925.