Quantifying the Physical Genome - William Greenleaf скачать в хорошем качестве

Quantifying the Physical Genome - William Greenleaf

The Greenleaf Lab aims to understand how a 2 meter long object, the human genome, encodes for molecules which, through physical interaction with the genome itself, evince the immense cellular diversity we observe in the human body. In this talk, Prof. Greenleaf discusses efforts to catalog regulatory elements and transcription factor grammars that are active during human fetal development, and describe single molecule approaches for quantitatively understanding the relationship between transcription factor binding sites, transcription factor occupancy, and gene expression in human cells. About William Greenleaf William Greenleaf is a Professor in the Genetics Department at Stanford University School of Medicine, with a courtesy appointment in the Applied Physics Department. He is a member of Bio-X, the Biophysics Program, the Biomedical Informatics Program, and Stanford Cancer Institute. He received an A.B. in physics from Harvard University in 2002, and received a Gates Fellowship to study computer science for one year at Trinity College, Cambridge, UK. He returned to Stanford to carry out his Ph.D. in Applied Physics in the laboratory of Steven Block, where he investigated, at the single molecule level, the chemo-mechanics of RNA polymerase and the folding of RNA transcripts. He conducted postdoctoral work in the laboratory of X. Sunney Xie in the Chemistry and Chemical Biology Department at Harvard University, where he was awarded a Damon Runyon Cancer Research Foundation Fellowship, and developed new fluorescence-based high-throughput sequencing methodologies. He moved to Stanford as an Assistant Professor in November 2011. Since beginning his lab, he has been named a Rita Allen Foundation Young Scholar, an Ellison Foundation Young Scholar in Aging (declined), a Baxter Foundation Scholar, a Chan-Zuckerberg Investigator, and Arc Institute Innovation Investigator, and received the NIH Director's Pioneer Award. His highly interdisciplinary research links molecular biology, computer science, bioengineering, and genomics a to understand how the physical state of the human genome controls gene regulation and biological state. Efforts in his lab are split between building new tools to leverage the power of high-throughput sequencing and cutting-edge microscopies, and bringing these new technologies to bear against basic biological questions of genomic and epigenomic regulation. His long-term goal is to unlock an understanding of the physical “regulome” — i.e. the factors that control how the genetic information is read into biological instructions — to develop a quantitative understanding of how cells maintain, or fail to maintain, their state in health and disease. Sponsored by the Center for Physical Genomics and Engineering, the Cancer and Physical Sciences Program at the Robert H. Lurie Comprehensive Cancer Center, and NIH Grants T32GM142604 and U54CA268084