Small Molecule Microarrays for Drug Discovery скачать в хорошем качестве

Small Molecule Microarrays for Drug Discovery

The speaker is Dr. Adam Buckle, Chief Scientific Officer at Arrayjet, hosted by Oleksii Gavrylenko, Chemspace Chief Operating Officer. We discussed the following topics related to the Small Molecule Microarray (SMM) methodology: What a SMM is, how they were developed, and how they are used; The full process of how SMM screening is carried out; The types of target molecules SMM can be used for, including case studies; How the technique addresses current drug discovery challenges. For more details please visit our website (https://tinyurl.com/24tjzubu) or drop us a line: sales@chem-space.com! #Chemspace #Arrayjet ____________________________________________________________________________________ If you would like to keep up on the latest news and updates, follow us on social media: LinkedIn:   / chemspace   Twitter:   / chem_space   Instagram:   / _chemspace_   Facebook:   / chemspace   0:00 Start of the webinar 2:40 Chemspace introduction by Oleksii Gavrylenko, Chief Operating Officer 9:52 Speaker introduction - Dr. Adam Buckle, CSO at Arrayjet 11:42 Introduction to Small Molecule Microarraying 11:58 Overview of the presentation 12:23 Arrayjet’s focus 12:59 Arrayjet products and services 13:51 What is a microarray? 14:43 What is a small molecule microarray? 15:30 Process overview: small molecule microarray. 16:05 Part 1. Small molecule libraries 18:08 Part 2. Isocyanate surface chemistry 20:00 Surface reactivity test for SM binding 22:26 Isocyanate surface chemistry: Why is it important? 23:33 Part 3. Printing: Mercury for Small Molecule Screening 24:16 Mercury only uses inkjet 24:53 Microfluidic sample transfer 25:40 A simplified view: aspiration and printing 26:24 We image every printed spot 26:51 Why Arrayjet and Small Molecule Microarraying (SMM) 28:58 Part 4. Binding assay and hits identification 29:11 Target types for screening assay – Protein and RNA 32:34 Protein SMM: Rapamycin example 34:02 Targeted protein degradation. PROTACs 37:03 Drugging RNA 38:58 Riboswitches as a Paradigm for understanding structural RNA 40:20 PreQ1-riboswitch SMM case study 42:04 Example SMM assay: PreQ1-riboswitch 43:26 Example SMM screen 44:30 Part 5. Validation. Target validation using SPR imaging 48:07 Benefits of SMM 50:34 Summary of SMM 50:42 Q&A session