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Join Anna, our talented intern at Plant Cell Technology, as she guides you through the essential steps to successfully tissue culture plants. This comprehensive tutorial covers everything from measuring and mixing to sterilizing and pouring media to ensuring your plant cultures thrive after acclimation. ⬇️ Timestamps ⬇️ 0:01 Intro to video on how to make plant tissue culture media 0:07 Method One - Pouring Before Autoclave 1:27 Media Sterilization Methods 2:01 Adding Gelling Agents - Agar 2:35 Method Two - Pouring In The Flow Hood 3:19 How to Autoclave The Media 3:58 Laminar Flow Hood Preparation 4:21 Removing The Media From The Autoclave 5:18 Pouring Media After Autoclaving 5:54 Flow Hood Aseptic Techniques Tips 6:39 Subculturing Tissue Culture Plants 8:07 Changing Liquid Media in The BioCouplers™ 8:26 BioCoupler™ Subculturing 8:56 How to Sterilize Plants For Tissue Culture 10:37 Plant Induction to Media 11:12 Plant Tissue Culture Acclimation to Soil 12:30 Outro 👉 Register for an upcoming Tissue Culture Master Class Here: https://plantcelltechnology.com/maste... Don't forget to subscribe and follow us on social media for more updates and tissue culture tips! 🌿 INSTAGRAM: / plantcelltech 🌿 FACEBOOK: / plantcelltechnology 🌿 LINKEDIN: / plant-cell-technology-inc- 🌿 TIKTOK: / plantcelltech 🌿 TWITTER: / plantcellnews 🛒 Products We Used in the Video: Supreme-Grade Agar: http://bit.ly/3T4nZy3 Gellan Gum: https://plantcelltechnology.com/produ... PPM™: http://bit.ly/3ZLhXES MS Media: https://plantcelltechnology.com/produ... Vented Test Tubes: https://plantcelltechnology.com/produ... Round Snap-Lock Containers: https://plantcelltechnology.com/produ... Biocouplers™: https://plantcelltechnology.com/produ... IBA Solution: http://bit.ly/3ZysQKA BAP Solution: https://www.plantcelltechnology.com/b... #TissueCulture #PlantScience #LabTechniques #AsepticTechnique #EducationalVideo #PlantCare #ScienceEducation #biotechnology Thanks for watching, and happy culturing!