Cell Culture Video: Step-by-Step Guide to Passaging Cells скачать в хорошем качестве

Cell Culture Video: Step-by-Step Guide to Passaging Cells

🔗 Learn more: https://www.thermofisher.com/global/e... This *cell culture video* provides a complete overview of passaging cells, an essential part of maintaining healthy cell cultures. By following these *cell culture training* steps, you’ll learn how to keep cells in optimal condition for experimental success. In this video, you’ll learn: The standard growth phases of cultured cells and when to passage. How to prepare your hood, flasks, and media while maintaining sterility. Step-by-step instructions for removing medium, rinsing, and applying dissociation reagents. How to confirm cell detachment using a microscope. Best practices for centrifugation, resuspension, and accurate cell counting. How to use trypan blue staining and automated cell counters for live/dead analysis. Tips for seeding new flasks at the correct density to maintain log-phase growth. These *tissue culture training* techniques ensure consistency and reduce contamination risks. Perfect for students, researchers, and anyone seeking a solid foundation in cell culture basics. 📌 Chapters: 00:00 – Introduction: Cell Growth and Culture Phases 00:40 – Preparing the Hood and Supplies 01:30 – Removing Medium and Washing Cells 02:10 – Adding Dissociation Reagents (TrypLE Express or Trypsin) 03:00 – Confirming Cell Detachment with a Microscope 04:00 – Centrifugation and Resuspension 05:00 – Cell Counting with Trypan Blue 06:00 – Seeding New Flasks and Maintaining Density 07:00 – Best Practices for Contamination Prevention #CellCulture