Basic adjustments of fluorescent images with FIJI скачать в хорошем качестве

Basic adjustments of fluorescent images with FIJI

Recommended: watch this video in HD for better text rendering. 00:00 Introduction 00:23 FIJI Installation 03:25 FIJI Updates 05:21 Opening fluorescence microscopy images 6:35 FIJI Bio-Formats Import options 9:00 Information in image title bar 10:22 Changing channel and Z planes in multidimensional datasets 11:19 Opening Brightness/Contrast and Channels tool 12:19 Channels tool: grayscale view, selecting display colors, composite view 15:50 Microscopy data is numbers associated with pixels that get displayed in different ways 20:37 Brightness/Contrast tool: adjusting how channels look 27:41 Getting images out of FIJI that are usable in other software 30:10 Adding a scale bar 34:03 Exporting individual channels 34:51 Putting everything together in a presentation 36:14 Working with more than one image: tile displayed windows, synchronize windows 39:49 Conditions for properly comparing multiple images 42:15 Propagate display settings across multiple images 45:31 Opening images from Zeiss microscopes in Zeiss software to easily access metadata 48:48 Guidelines for writing proper materials and methods: https://www.med.unc.edu/microscopy/ri... 49:07 Acknowledge the Microscopy Services Laboratory: https://www.med.unc.edu/microscopy/us...