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📌 Description: 🔬 Welcome to Lecture 21 of the Sci-Chat Mindset tutorial series — your go-to platform for insightful and practical bioinformatics learning! In this lecture, we dive deep into iTOL (Interactive Tree of Life) — a user-friendly and highly customizable web-based tool for phylogenetic tree visualization, annotation, and interpretation. This tutorial is specially designed for students and researchers in microbiology, metagenomics, evolutionary biology, and comparative genomics who want to enhance the visual clarity and analytical depth of their phylogenetic data. 🌿 Whether you're analyzing 16S rRNA-based microbial diversity, whole genome comparisons, or exploring evolutionary relationships, iTOL allows you to upload your trees (e.g., Newick format) and visually annotate them with rich metadata layers — such as heatmaps, colored strips, bar charts, or gene presence/absence matrices. 🎯 What You’ll Learn in This Lecture: How to access the iTOL platform and navigate its interface Step-by-step guide to uploading phylogenetic tree files (Newick format) How to customize layout styles: circular, unrooted, radial, and more Techniques for adding and managing metadata annotations (e.g., taxonomy, resistance genes, functional classes) Coloring clades and branches, adding bootstrap values, and editing labels Exporting publication-quality trees in various formats (PNG, SVG, PDF) 🎓 This session empowers you to present your phylogenetic data visually and intuitively — making your research not only scientifically robust but also visually appealing for presentations and publications. 🔗 Don’t forget to check previous lectures on Galaxy, NCBI SRA, MAFFT, and BPGA if you're working on comparative genomics, metagenomic analysis, or functional profiling. 👍 If you find this helpful, don’t forget to Like, Comment, and Subscribe to Sci-Chat Mindset for more tutorials on cutting-edge biological data tools and techniques! #iTOL #InteractiveTreeOfLife #Phylogenetics #Bioinformatics #SciChatMindset #PhylogeneticVisualization #GenomeAnalysis #TreeAnnotation #MicrobialGenomics #EvolutionaryBiology #NGSAnalysis #ComparativeGenomics #FunctionalGenomics #Metagenomics