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Watch the Full Video at https://www.jove.com/v/50294/purifica.... Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media - a 2 minute Preview of the Experimental Protocol Marguerite K. McDonald, Kathryn E. Capasso, Seena K. Ajit Drexel University College of Medicine, Department of Pharmacology & Physiology; The presence of stable microRNAs (miRNAs) in exosomes has generated immense interest as a novel mode of intercellular communication, for their potential utility as biomarkers and as a route for therapeutic intervention. Here we demonstrate exosome purification from blood and culture media followed by quantitative PCR to identify miRNAs being transported. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments