Alcian Blue stain to detect acidic polysaccharides glycosaminoglycans and mucopolysaccharide скачать в хорошем качестве

Alcian Blue stain to detect acidic polysaccharides glycosaminoglycans and mucopolysaccharide

Alcian Blue is a histological stain used to detect acidic mucopolysaccharides and glycosaminoglycans, commonly found in mucins and cartilage. It is highly valued in pathology for identifying tissues rich in acid mucins. Key Features of Alcian Blue Stain: Specificity for Acid Mucins: Alcian Blue binds to acidic components in tissues, such as sulfated and carboxylated mucopolysaccharides. Color Reaction: It stains these substances in shades of blue, which contrasts well with counterstains like Nuclear Fast Red, used to highlight nuclei. Applications: Mucin Detection: Identifying mucinous tumors, such as adenocarcinomas, that secrete acid mucins. Cartilage Analysis: Staining cartilage matrix to study its structural composition. Disease Diagnosis: Detecting conditions involving excessive mucin production, like certain gastric or pulmonary disorders. Variants: pH Specificity: At pH 2.5, it detects both sulfated and carboxylated acid mucins, whereas at pH 1.0, it selectively stains sulfated mucins. #glycosaminoglycan