What is a kymograph in single molecule analysis? скачать в хорошем качестве

What is a kymograph in single molecule analysis?

The kymograph. One of the most information-rich and layered data representations available for single-molecule researchers. In the eye of some beholders, there’s even a beauty to it. With a new educational animation short, we explore the full basic powers of the kymograph utilized to clearly visualize C-Trap data. We also explain how to read a kymograph. What is the kymograph? And how does it visualize molecular interactions? Simply put, a kymograph shows how individual molecules move over time. First, a single DNA molecule is immobilized in solution with the help of optical traps. The DNA is then allowed to interact with fluorescently labeled proteins in the sample. Performing confocal line scans along the DNA delivers snapshots of the proteins interacting with the DNA at that specific time. In the C-Trap, this is repeated up to 100 times per second. Over time, each protein interacting with the DNA creates a fluorescence trace in the kymograph. From the kymograph, we can derive: 🎯 Binding location or sequence 🚦 Binding and unbinding rates ⏲️ Duration of the interaction 📏 Diffusion coefficient 🪃 Rate of directed movement See how all that works in the animation.