Calum Walsh – Rational Engineering of Live Bacterial Therapeutics from a Human Biobank | MVIF41 S05 скачать в хорошем качестве

Calum Walsh – Rational Engineering of Live Bacterial Therapeutics from a Human Biobank | MVIF41 S05

Title of the presentation: Rational Engineering of Live Bacterial Therapeutics from a Human Microbiome Biobank Speaker: Calum Walsh More info on this event: https://www.microbiome-vif.org/en-US/... Join us at the next meeting (free registration!): https://www.microbiome-vif.org/ And you can join us on Twitter:   / microbiomevif   Abstract of this talk: Multidrug-resistant organisms (MDROs) frequently colonise the gut, limiting treatment options. Here, we present the Centre for Pathogen Genomics Microbiome Culture Collection (CMC), a greater than 2,000-isolate, genomically characterised biobank of human gut commensals supporting the rational design of live bacterial therapeutics (LBTs). Leveraging genome-level resolution to assess safety and functional potential, we assemble small, complementary consortia intended to restore colonisation resistance and disrupt MDRO niches, with carbapenem-resistant Klebsiella pneumoniae (CRE-Kp) as a priority target. Using in vitro and in vivo assays, we show that rationally designed consortia reproducibly suppress CRE-Kp growth, with several combinations driving rapid decline of pathogen abundance and preventing rebound. These findings illustrate how genomically-resolved culture resources can be translated into mechanism-informed, precision LBT candidatesm with the CMC serving as a valuable resource for accelerating MDRO decolonisation strategies by linking isolate-level genomics with consortium design and functional testing against high-priority threats.