Degraded FFPE-RNA Samples Sequencing and Analysis | Protocol Preview скачать в хорошем качестве

Degraded FFPE-RNA Samples Sequencing and Analysis | Protocol Preview

Watch the Full Video at https://www.jove.com/v/61060/optimiza.... Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples - a 2 minute Preview of the Experimental Protocol Yelena Levin, Keyur Talsania, Bao Tran, Jyoti Shetty, Yongmei Zhao, Monika Mehta Frederick National Laboratory for Cancer Research, NCI CCR Sequencing Facility; Frederick National Laboratory for Cancer Research, Advanced Biomedical and Computational Sciences; This method describes the steps to improve the quality and quantity of sequence data that can be obtained from formalin-fixed paraffin-embedded (FFPE) RNA samples. We describe the methodology to more accurately assess the quality of FFPE-RNA samples, prepare sequencing libraries, and analyze the data from FFPE-RNA samples. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments