RNaseH sequences preferences affecting antisense RNA drugs and HIV replication - Jeppe Vinther скачать в хорошем качестве

RNaseH sequences preferences affecting antisense RNA drugs and HIV replication - Jeppe Vinther

RNase H cleaves RNA in RNA-DNA duplexes. It is present in all domains of life as well as in multiple viruses and is essential for mammalian development and for HIV replication. Moreover, human RNase H1 is essential for the therapeutic effect of DNA-like antisense oligonucleotides. Here, I will our efforts to understand the sequence preferences of HIV-1, human and E. coli RNase H enzymes using sequencing-based methods. Our findings can potentially be used to improve the design of RNase H-recruiting antisense oligonucleotides and show that sequence preferences of HIV-1 RNase H have shaped evolution of the HIV-1 genome and may contribute to the conserved use of tRNA-Lys3 as primer during viral replication. -- Danish Institute for Advanced Study (D-IAS) is a national elite center at University of Southern Denmark (SDU) that sets the framework for excellent research. We offer an extensive program of different prestigious lectures and we aim to inspire groundbreaking ideas through the meeting of minds within all disciplines. D-IAS include chairs from all five faculties at SDU and three external chairs from the University of Copenhagen. The most important core in D-IAS we believe is our team of 14 very promising D-IAS Assistant Professors.