Methods to determine genotype or genotyping - Tutorial скачать в хорошем качестве

Methods to determine genotype or genotyping - Tutorial

This video covers the definition of genotype and genotyping, as well as descriptions of several methods of genotyping. Among the methods covered to ascertain the genotype for a particular phenotype are pedigree data, PCR followed by electrophoresis including T-ARMS-PCR or tetra-primer amplification refractory mutation system—PCR, BeadChips, and PCR followed by sequencing. These methods will allow the genotyping of different alleles, including length variants and SNPs. At the end of the video is a Q&A section to reinforce the concepts covered. Chapters 00:00 What are genotypes? 00:26 An example of the need for genotyping. 00:56 Genotype predicted from phenotypes of family members 01:38 Genotypes obtained from DNA 03:02 Genotyping alleles of different lengths using PCR followed by electrophoresis 05:16 Genotyping SNPs using T-ARMS PCR 06:36 Genotyping SNPs using BeadChips 08:06 Genotyping using PCR followed by sequencing 08:19 Summary 08:58 Questions and Solutions