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Watch the Full Video at https://www.jove.com/v/51648/a-new-cl.... A New Clarification Method to Visualize Biliary Degeneration During Liver Metamorphosis in Sea Lamprey (Petromyzon marinus) - a 2 minute Preview of the Experimental Protocol Yu-Wen Chung-Davidson, Peter J. Davidson, Anne M. Scott, Erin J. Walaszczyk, Cory O. Brant, Tyler Buchinger, Nicholas S. Johnson, Weiming Li Michigan State University, Department of Fisheries & Wildlife; U.S. Geological Survey, Hammond Bay Biological Station, Great Lakes Science Center; Sea lamprey lose the gall bladder and bile ducts during metamorphosis, a process similar to human biliary atresia. A new fixation and clarification method (CLARITY) was modified to visualize the entire biliary tree using laser scanning confocal microscopy. This method provides a powerful tool to study biliary degeneration. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments