Sequential Photobleaching: Visualization with GFP in Schwann Cells | Protocol Preview скачать в хорошем качестве

Sequential Photobleaching: Visualization with GFP in Schwann Cells | Protocol Preview

Watch the Full Video at https://www.jove.com/v/4460/sequentia.... Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction - a 2 minute Preview of the Experimental Protocol Monika S. Brill, Petar Marinković, Thomas Misgeld Technische Universität München, Lehrstuhl für Biomolekulare Sensoren; Technische Universität München, Center for Integrated Protein Science (Munich) at the Institute of Neuroscience; Technische Universität München, TUM Institute for Advanced Study and German Center for Neurodegenerative Diseases; Technische Universität München, Munich Cluster for Systems Neurology (SyNergy); Visualizing individual cells in densely packed tissues, such as terminal Schwann cells (SCs) at neuromuscular junctions (NMJs), is challenging. "Sequential photo-bleaching" allows delineating single terminal SCs, for instance in the triangularis sterni muscle explant, a convenient nerve-muscle preparation, where sequential bleaching can be combined with time-lapse imaging and post-hoc immunostainings. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments