EYRAplex tutorial: step-by-step full assay protocol скачать в хорошем качестве

EYRAplex tutorial: step-by-step full assay protocol

Learn how to perform an EYRAplex multiplex assay with Mabtech in this step-by-step tutorial video. Follow along as we go through the entire protocol with a number of helpful tips along the way. 00:00 Prepare your materials 01:26 Reconstitute standard and prepare reagents 02:12 Standard dilutions 03:02 Add Capture bead mix to the plate and wash 04:03 How to wash the EYRAplex plate with a handheld magnetic plate washer 04:57 Add standards and diluted samples and incubate for 2 hours 05:59 Prepare and filter the Detection mAb mix and wash the plate 07:09 Add the Detection mAb mix and incubate for 1 hour 07:42 Prepare the SA-PE and wash the plate 08:23 Add the SA-PE and incubate for 30 minutes 09:04 Final wash step and prepare for analysis in Mabtech EYRA or on a flow cytometer About EYRAplex multiplex assays: EYRAplex is a magnetic bead-based multiplex assay for the simultaneous detection and quantification of multiple analytes in a single sample. The magnetic beads have been dyed with different concentrations of two fluorophores, resulting in distinct bead populations with unique bead IDs. Each bead population has been conjugated with a specific monoclonal antibody, allowing specific binding to its corresponding analyte. Multiplex analysis is enabled by combining several bead populations that are supplied as a capture bead mix. In EYRAplex, the capture bead mix is added to wells in a 96-well plate. Samples and standards are then added. During incubation, the capture antibodies bind to analytes present in the sample. Detection occurs in two steps. First, a biotin-labeled monoclonal antibody mix (detection mAb mix) is added. These detection antibodies bind to the captured analytes. Streptavidin-PE is then added, binding to the biotin and generating a fluorescent signal proportional to the analyte concentration. The fluorescent signal from each bead ID determines the analyte, while the streptavidin-PE signal reflects its quantity. Analytes are quantified by comparing the signals to standard curves. Learn more at mabtech.com