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Watch the Full Video at https://www.jove.com/v/60795/intrathe.... Intrathecal Application of a Fluorescent Dye for the Identification of Cerebrospinal Fluid Leaks in Cochlear Malformation - a 2 minute Preview of the Experimental Protocol Nora M. Weiss, Ingo Andus, Armin Schneider, Sönke Langner, Stefanie Schröder, Sebastian P. Schraven, Robert Mlynski University Medical Center Rostock, Department of Otorhinolaryngology, Head and Neck Surgery; Rostock University,; ARRI Medical GmbH,; University Medical Center Rostock, Department of Radiology and Institute of Diagnostic and Interventional Radiology; Intrathecally applied fluorescein is used to achieve intraoperative visualization of CSF leaks. This protocol describes a lumbar puncture, the application of 5% fluorescein, and intraoperative visualization using a fully digital microscope. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments