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General Troubleshooting for Chemi Blots There are several steps that can be optimized, from the amount of loaded protein, through transfer conditions, all the way down to the substrate used to visualize your blots. Don’t forget that simple things like length and conditions of your primary antibody incubation as well as washing steps can have a big impact on your final data. Your antibody and substrate data sheets are a great resource to help you troubleshoot and optimize your Western blot protocol. About LICORbio LICORbio offers imaging systems, analysis software, and IRDye® infrared dye reagents for quantitative protein and small animal imaging. LICORbio provides an integrated solution for quantitative Western blots with its Odyssey® Infrared Imaging Systems, Image Studio™ quantification software, Empiria Studio® analysis software for publication-ready data, reagents, unique IRDye® secondary antibodies, and online training courses. For chemiluminescent Western blots, the C-DiGit® Blot Scanner is a personal, portable imager. Use the Odyssey® XF or Odyssey M Imaging Systems for both chemiluminescence and near-infrared fluorescence Western blots. You can achieve superior small animal imaging results using BrightSite™ near-infrared fluorescent probes or bioluminescence on the Pearl® Trilogy imaging system. LICORbio Website https://www.licorbio.com/ Reagent Store https://www.licor.com/bio/reagents Image Studio™ Software https://www.licor.com/image-studio LinkedIn / licorbio Twitter / licorbio Facebook / licorbio