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Plasmids are so useful because of their flexibility. But how does the plasmid express the gene of interest? For an answer to that, watch our video on the Promoter Region. https://blog.addgene.org/plasmids-101... Our Plasmids 101 videos are designed to educate all levels of scientists and plasmid lovers. For more, https://blog.addgene.org/topic/plasmi... __________________________________________________________________________ 0:00 - Intro 0:33 - Promoter Region Overview 1:48 - Regulatory Element: Enhancer 2:10 - Regulatory Element: Silencer 2:33 - Regulatory Element: Insulator 2:55 - Considerations on Choosing a Promoter 3:55 - Leaky Transcription 4:30 - Synthetic Promoters 4:45 - Outro __________________________________________________________________________ Credits: Narrated by: Aliyah Weinstein Written, Directed & Animated by: Quintin Marcelino Based on Previous Material by: Kendall Morgan Designs and Illustrations by: Jason Snair Additional Designs by: Quintin Marcelino Sound Design & Sound Editing by: Quintin Marcelino. Music by: Squadda B and The Grand Affair, courtesy of Youtube Audio Library Special Thanks to: Jennifer Tsang, Aliyah Weinstein, Brook Pyhtila, Seth Kasowitz, Mike Lacy, Steven DiPrizio, and everyone at Addgene who provided support and feedback on this project