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Reference: https://app.jove.com/v/1662/using-gen... The Gene Pulser MXcell Electroporation System is a cutting-edge tool designed to facilitate the efficient transfection of primary cells, which are often challenging to manipulate due to their unique characteristics and requirements. This advanced system utilizes electroporation technology, a method that applies an electrical field to cells, temporarily permeabilizing their membranes. This process allows for the introduction of various molecules, such as plasmid DNA, RNA, or proteins, into the cells with high efficiency. One of the key advantages of the Gene Pulser MXcell system is its versatility, as it can be used with a wide range of cell types, including hard-to-transfect primary cells derived from tissues such as blood, brain, and muscle. The system is equipped with customizable parameters, allowing researchers to optimize the electroporation conditions for specific cell types and experimental needs. This flexibility is crucial for achieving high transfection rates while minimizing cell damage. Additionally, the Gene Pulser MXcell system is designed with user-friendly features, including an intuitive interface and pre-set protocols for various cell types, making it accessible for both experienced researchers and those new to the field of cell transfection. The system also supports high-throughput applications, enabling researchers to conduct multiple experiments simultaneously, which can significantly accelerate the pace of research and development.