Aortic Valve Calcification Investigating by Feeder Cells | Protocol Preview скачать в хорошем качестве

Aortic Valve Calcification Investigating by Feeder Cells | Protocol Preview

Watch the Full Video at https://www.jove.com/v/62059/investig.... Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat - a 2 minute Preview of the Experimental Protocol Lavinia Curini, Mary Roxana Christopher, Herko Grubitzsch, Ulf Landmesser, Amedeo Amedei, Alexander Lauten, Brunilda Alushi Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), Department of Cardiology, Campus Benjamin Franklin; University of Florence, Department of Experimental and Clinical Medicine; Berlin Institute of Health,; German Heart Centre Berlin (DHZB), Department of Cardiology; Azienda Ospedaliera Universitaria Careggi (AOUC), Sod of Interdisciplinary Internal Medicine; Helios Klinikum Erfurt, Department of General and Interventional Cardiology; In this study, we describe the process of T lymphocyte isolation from fresh samples of calcified aortic valves and the analytical steps of T cell-cloning for the characterization of the adaptive leukocyte subsets by using flow cytometry analysis. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments