QDB Analysis to Determine Selected Protein at Tissue Level | Protocol Preview скачать в хорошем качестве

QDB Analysis to Determine Selected Protein at Tissue Level | Protocol Preview

Watch the Full Video at https://www.jove.com/v/56885/high-thr.... High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB) - a 2 minute Preview of the Experimental Protocol Xiaoying Qi, Yunyun Zhang, Yuan Zhang, Tianhui Ni, Wenfeng Zhang, Chunhua Yang, Jia Mi, Jiandi Zhang, Geng Tian Binzhou Medical University, Medicine and Pharmacy Research Center; Yantai Zestern Biotechnique Co. LTD,; Binzhou Medical University, Precision Medicine research center; Uppsala University, Department of Chemistry - BMC; Here we demonstrate a detailed process of quantitative dot blot analysis (QDB) by determining the absolute content of a targeted protein, capping actin protein, gelsolin-like (CAPG), in three different mouse tissues. We demonstrate a high throughput, convenient, quantitative immunoblot technique for biomarker validation at the cellular and tissue level. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments