Extracellular vesicles characterization and isolation through nanoscale flow cytometry скачать в хорошем качестве

Extracellular vesicles characterization and isolation through nanoscale flow cytometry

Presented By: Professor Claudia Maria Radu Webinar: Extracellular vesicles characterization and isolation through nanoscale flow cytometry Webinar Abstract: Extracellular Vesicles (EVs) are key mediators of intercellular communication, enclosed by a phospholipid bilayer and lack the ability to replicate. Characterization of EVs is essential to elucidate their cellular origins, molecular cargo, and functional roles in health and disease. However, the nanoscale size and low refractive index (RI) present significant challenges for detection and size determination with conventional flow cytometers. Furthermore, the use of standard polystyrene beads for calibration complicates the analysis due to their significantly higher light-scattering properties compared to EVs. The CytoFLEX nano represents a new platform for EV evaluation. The instrument is equipped with a multiple-scatter laser which increases the sensitivity for the detection of small EVs. By combining scatter-based detection with fluorescence antibodies labelling, size, concentration, and expression of EVs surface markers can be measured simultaneously, providing a more accurate and sensitive approach to EVs analysis. Labroots on Social: Facebook:   / labrootsinc   Twitter:   / labroots   LinkedIn:   / labroots   Instagram:   / labrootsinc   Pinterest:   / labroots   SnapChat: labroots_inc