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Watch the Full Video at https://www.jove.com/v/54065/a-mouse-.... A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure - a 2 minute Preview of the Experimental Protocol Matthew J. Hartsock, Hongkwan Cho, Lijuan Wu, Wan-Ju Chen, Junsong Gong, Elia J. Duh Johns Hopkins University, Department of Ophthalmology, School of Medicine; This article describes a procedure for inducing retinal ischemia-reperfusion injury by elevated intraocular pressure in mice. Retinal ischemia-reperfusion injury by elevated intraocular pressure serves to model human pathologies characterized by compromised oxygen and nutrient delivery in the retina, enabling researchers to examine potential cellular mechanisms and treatments for human diseases of the retinal neurovascular unit. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments