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Watch the Full Video at https://www.jove.com/v/3855/optical-f.... Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation - a 2 minute Preview of the Experimental Protocol Lida P. Hariri, Matthew B. Applegate, Mari Mino-Kenudson, Eugene J. Mark, Brett E. Bouma, Guillermo J. Tearney, Melissa J. Suter Harvard Medical School, Department of Pathology; Massachusetts General Hospital,; Harvard Medical School, Wellman Center for Photomedicine; Massachusetts General Hospital, Pulmonary and Critical Care Unit; Harvard Medical School, Pulmonary and Critical Care Unit; A method to image ex vivo pulmonary resection specimens with optical frequency domain imaging (OFDI) and obtain precise correlation to histology is described, which is essential to developing specific OFDI interpretation criteria for pulmonary pathology. This method is applicable to other tissue types and imaging techniques to obtain precise imaging to histology correlation for accurate image interpretation and assessment. Imaging criteria established with this technique would then be applicable to image assessment in future in vivo studies. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments